Timm’s 铜染色法固定：福尔马林固定或新鲜组织技术方法：石蜡切片或冰冻切片试剂：液体A ：5%硝酸银水溶液液体B：对苯二酚2.0g， 柠檬酸5.0g， 蒸馏水100ml ，液体可以贮存在4℃暗处一个月。步骤：1．石蜡切片脱蜡至水2．0.5%硫化铵5min3．充分水洗4．用0.1N盐酸2-3min祛除铁和硫化锌液（15%三氯醋酸也 ...

DAPI staining yeast[*]1mL yeast + 0.1mL 36% formaldehyde. 1-2H/Rt [*]Wash 2x 1mL H2O (Spin at 10K, 5" ok). May leave at 4oC [*]To stain: Spind down and resuspend in 300 ul H2O. Add 700 ul 100% EtOH. Leave for 30 min/Rt. [*]Spin down, resuspend 500 ul H2O and sonicate 5 sec. [*]Spin down (10K, 5") an ...